Wrapper for bin_interactions() that interacts with the filesystem

Places putative interactions into equally-sized bins based on the distances they span, and stores these on disk.

bin_interactions_fs(
  interactions_file,
  fragments_file,
  output_dir,
  min_dist = 2500,
  max_dist = Inf,
  bins = 5
)

Arguments

interactions_file	Path to (tsv or csv) file containing putative interactions. Its first line must state the columns names: baitID, preyID, N. Subsequent lines report one putative interaction each: a bait fragment ID, prey fragment ID and readcount.
fragments_file	Path to (bed) file containing fragment information. Its first line must state the column names: chrom, chromStart, chromEnd, ID. Each subsequent line reports the chromosome, starting coordinate, ending coordinate and ID of a fragment.
output_dir	Directory where the generated bins will be stored. Will be created if it does not exist.
min_dist	Minimum distance interactions must span to be included in the analysis. Distance is defined between fragment midpoints.
max_dist	Maximum distance interactions can span to be included in the analysis. Distance is defined between fragment midpoints.
bins	Number of bins to place the interactions into.

Value

List containing the output directory and an overview of bins.

Examples

base = system.file("extdata",package="peaky")

interactions_file = paste0(base,"/counts.tsv")
fragments_file = paste0(base,"/fragments.bed")
bins_dir = paste0(base,"/bins")
if (FALSE) {
BI = bin_interactions_fs(interactions_file, fragments_file, output_dir=bins_dir)
print(BI)
}